Hua
HEPES konutai tote
Nga kupu taurite:HEPES konutai tote;75277-39-3;HEPES hemisodium tote;103404-87-1;Konutai 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;konutai 2-[4-(2-hydroxyethyl) )piperazin-1-yl]ethanesulfonate;HEPES (konutai);C8H17N2NaO4S;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium tote;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, tote monosodium;4-(2-Hydroxyethyl)piperazine-1 te waikawa ethanesulfonika konutai tote; 4-(2-hydroxyethyl)piperazin-1-ylethanesulphonate;UNII-Z9FTO91O8A;Z9FTO91O8A;konutai;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate;DTXSID4044458;CHEBI:46758 278-169-7;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, konutai tote (1:1);N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, konutai tote;HEPES konutai te tote, >=99.5% (titration); konutai hepes;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) konutai tote;MFCD00036463;HEPES, konutai tote;HEPES konutai tote, 98%;C8H18N2O4S.Na;SCHEMBL229142;WAS-14;CHEMBL3187284;DTXCID2024458;RDZTWEVXRGYCFV-UHFFFAOYSA-M;C8-H18-N2-O4-S.Nalt sodium salt;HEPES. (1M);Tox21_302155;HB5187;N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonicacid)hemisodiumtote;HEPES konutai tote, matū koiora kōeke;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-0610868;F2A868; N'-hydroxyethyl-N-piperazineethanesulfonate;HEPES konutai tote, BioXtra, >=99.0% (titration);HEPES konutai tote, Vetec(TM) kōeke reagent, 96%;W-104397;Q27120698;Sodium2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid konutai tote;4-(2-hydroxyethyl)- 1-piperazineethanesulfonic waikawa, monosodium tote;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)sodiumtote;N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium tote;HEPES konutai tote, BioPerformance Certified, e tika ana mo te ahurea pūtau, >=99.5%
Taonga matū o HEPES konutai Tote
● Te ahua/Tae:paura ma
● Kohu Kohu: 0Pa i te 25 ℃
● Ira Whakarewa:234 °C
● PKA:7.5(i te 25 ℃)
● PSA：92.29000
● Kiato:1.504[i te 20℃]
● Rangitaki:-0.90190
● Rokiroki Temp.:Poko ki RT.
● Tairongo.:Hygroscopic
● Wairewa.:H2O: 1 M i te 20 °C, maamaa, kore tae
● Wairewa Wai.:Ka whakarewahia ki te wai.
● Tatau Kaihoko Hiko Hauwai:1
● Tatau Kaikawe Paihere Waiwai:6
● Tatau here ka taea te huri:5
● Papatipu Tika:260.08067248
● Tatau ngota Taumaha:16
● Matatini:272
Nga korero haumaru
● Pikito/ngā:
● Waehere morearea:
● Nga Korero Haumaru:22-24/25
Whai hua
Karaehe matū:Ētahi atu Whakamahinga -> Biological Buffers
NGĀ ATA KANOI:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Whakamahinga:He pai te otinga tote konutai HEPES mo nga rangahau ahurea pūtau. Ka taea pea te whakamahi hei whakarite i te otinga parepare o HEPES. Ka taea pea te whakamahi hei parepare i te wa o nga whakamatautau phosphorylation i roto i nga fibroblasts permeabilized. Ka taea pea te whakamahi hei tito i tetahi kaitarai mate mate mate, ka whakamahia hei whakatau i te thyroxin kore utu i roto i te serum ma te radioimmunoassay. He pai te wairewa tote konutai HEPES hei whakamahi i roto i nga rangahau ahurea pūtau. Ka taea pea te whakamahi hei whakarite i te otinga parepare o HEPES. Ka taea te whakamahi hei parepare i te wa e whakamatautau ana i te phosphorylation i roto i nga fibroblasts permeabilized. Ka taea pea te whakamahi hei tito i tetahi kaitarai mate mate mate, ka whakamahia hei whakatau i te thyroxin kore utu i roto i te serum ma te radioimmunoassay.
Whakaaturanga Taipitopito
HEPES konutai tote, e mohiotia ana ko 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid konutai tote, he momo HEPES e whakamahia nuitia ana. He puhui waro zwitterionic e mahi ana hei kaikawe i roto i te rangahau koiora me te rangahau matū koiora.
He maha nga wa ka whakamahia te tote konutai HEPES i roto i nga papaaaahuaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa e eeeteeetee e te kaha ki te pupuri i te awhe pH pumau huri noa i ngaaaahuatanga tinana (pH 7.2 - 7.6). He tino wairewa i roto i te wai, he iti te paitini, e pai ana mo nga momo tono.
Ko te ahua tote konutai o HEPES he pai ake i roto i te maha o nga tono na te mea ka whakarei ake i te wairewa me te pumau, ka whakaritea ki te ahua waikawa kore utu. E wātea ana ki te hokohoko hei paura tioata ma e ngawari ana te rewa ki te wai hei whakarite i nga otinga mahi.
Ka whakamahia e nga Kairangahau te tote konutai HEPES i roto i nga tikanga koiora ngota, te ahurea pūtau, te whakamaarama i te enzyme, te purenga pūmua, me etahi atu whakamatautau matū koiora he mea nui te whakahaere pH. Ko te kaha ki te pupuri me te hototahi ki nga punaha koiora he mea rongonui mo te pupuri i nga tikanga tino pai mo te mahi me te pumau o nga momo ngota koiora.
I mua i te whakamahi i te tote konutai HEPES i roto i tetahi tono motuhake, he mea nui ki te arotake i nga tuhinga, ki te tirotiro i nga taunakitanga a nga kaiwhakarato, me te arotau i te kukū me te awhe pH i runga i nga whakaritenga whakamatautau motuhake.
Taupānga
Ko te tote konutai HEPES he nui te tono ki nga waahi rangahau koiora me te matū koiora. Ko etahi o ana whakamahinga matua ko:
Tikanga Pūtau:Ko te tote konutai HEPES ka taapirihia ki nga papaaaahuaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa teaaaaaaa e ngaa puutau te tipu me te tipu.
Kaipupuri:He maha nga wa e whakamahia ana te tote konutai HEPES hei kaikawe parepare i roto i nga kaitarai koiora me nga otinga, tae atu ki te whakamatautau i te whanariki, te purenga pūmua, me nga tikanga koiora ngota. Ka awhina i te pupuri i te pH pumau ma te aukati i nga huringa o te waikawa me te kawakore.
Electrophoresis: Ka whakamahia te tote konutai HEPES hei parepare i roto i te electrophoresis gel hei pupuri i te pH pumau i te wa e wehea ana nga waikawa karihi, nga pūmua ranei. Ka aukati i nga nekehanga i roto i te pH ka pa ki te hekenga me nga tauira wehenga o nga ngota i roto i te ra.
Pūmautanga Pūmua: Ka taapirihia te tote konutai HEPES ki nga otinga pūmua hei whakapumau i te hanganga me te pupuri i a raatau mahi. Ka awhina i te pupuri i te taiao pH tino pai e tika ana mo te pumau me te mahi o te pūmua.
Mahi Enzyme: Ka whakamahia te tote konutai HEPES hei parepare i roto i nga tauhohenga enzymatic ki te pupuri i te pH e hiahiatia ana mo te mahi pai o te enzyme. Ka awhina i te whakarite kia mahi nga enzymes i te tino pai me te tika.
Atahanga Pūtau Ora:He maha nga wa ka whakaurua te tote konutai HEPES ki roto i te whakamaarama whakaahua mo nga whakamatautau whakaahua whakaahua. Ko tana kaha ki te pupuri i te pH e hiahiatia ana, ka aukati i nga rereketanga ka pa ki te kaha me te rere o nga pūtau.
Tikanga Koiora Molecular: He maha nga mahi a te tote konutai HEPES i roto i nga momo tikanga koiora ngota, tae atu ki te wehenga DNA, RNA ranei, PCR, raupapa DNA, me te tātari pūmua. Ko tana kaha ki te pupuri i nga hua tika me te pono i roto i enei mahinga.
He mea nui kia mohio he rereke pea te tono me te kukū o te tote konutai HEPES i runga i nga whakaritenga whakamatautau me te punaha koiora e rangahaua ana. Na reira, he mea tika kia tirohia nga tuhinga me nga taunakitanga a nga kaiwhakarato mo te whakamahi tino pai.
